| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 561 | Leptospira biflexa | AAGCCAGTATGCGCACCA | GCGAACCTTGTGCCTTTGT | 59.65 | 59.27 | 276 | 102 |
100.00%
|
100.00%
|
| Primer 562 | Leptospira biflexa | CGTTGAGTAGCCAAGGCCA | ACCATCCATCAGCACCCTC | 60.00 | 59.08 | 249 | 102 |
100.00%
|
100.00%
|
| Primer 563 | Leptospira biflexa | TGGGGTGAACGAGCCTTTC | ACACCACCAATCGCACCA | 59.93 | 59.49 | 296 | 102 |
100.00%
|
100.00%
|
| Primer 564 | Leptospira biflexa | CCGCATTCCTTGGTGGGAT | ACACCACCAATCGCACCA | 60.08 | 59.49 | 259 | 102 |
100.00%
|
100.00%
|
| Primer 565 | Leptospira biflexa | AAGTGCCGAGGTACTTGCG | AGGTTGCACCAGCTTTTGC | 60.37 | 59.56 | 212 | 102 |
100.00%
|
100.00%
|
| Primer 566 | Leptospira biflexa | GCCAAACGAAGGCCAACAG | TCGTTTTCCGTCACGCCA | 60.01 | 59.89 | 170 | 102 |
100.00%
|
100.00%
|
| Primer 567 | Leptospira biflexa | TGCGCTGATAATTTGCCCA | TGGATGCATTCACCAGGACA | 58.12 | 59.30 | 155 | 102 |
100.00%
|
100.00%
|
| Primer 568 | Leptospira biflexa | AGGTGTTGCAAGCGGATCA | CACTTGGACCACAAACGCG | 59.93 | 60.01 | 242 | 102 |
100.00%
|
100.00%
|
| Primer 569 | Leptospira biflexa | ACGAAGCCCTTGTGACCTG | ACCTGGTCTTGGCTCACAC | 59.93 | 59.55 | 202 | 102 |
100.00%
|
100.00%
|
| Primer 570 | Leptospira biflexa | TCGCCTGTTCATCACCATCC | TGGGCAAAGATCCACACCA | 60.11 | 59.15 | 287 | 102 |
100.00%
|
100.00%
|